- Description: thiamine ABC transporter (binding protein)
Gene name thiU Synonyms ykoF Essential no Product thiamine ABC transporter (binding protein) Function thiamine uptake Gene expression levels in SubtiExpress: thiU Interactions involving this protein in SubtInteract: ThiU Metabolic function and regulation of this protein in SubtiPathways: thiU MW, pI 21 kDa, 6.046 Gene length, protein length 600 bp, 200 aa Immediate neighbours thiV, ykoG Sequences Protein DNA DNA_with_flanks Genetic context Expression at a glance PubMed
ABC transporters, biosynthesis of cofactors, membrane proteins
Thi-box
Basic information
- Locus tag: BSU13240
Phenotypes of a mutant
Database entries
- BsubCyc: BSU13240
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- ThiW-(ThiV-ThiX)-ThiU PubMed
- Localization: membrane associated (via ThiV-ThiX) PubMed
Database entries
- BsubCyc: BSU13240
- Structure: 1SBR (complex with thiamine), 1S99
- UniProt: O34911
- KEGG entry: [2]
- E.C. number:
Additional information
- Operon: thiU-thiV-thiW-thiX PubMed
- Expression browser: thiU PubMed
- Sigma factor:
- Regulation:
- repressed by thiamine (Thi-box) PubMed
- Regulatory mechanism:
- Thi-box: RNA switch PubMed
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 257 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 745 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1463 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 433 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 540 PubMed
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis. J Bacteriol: 2005, 187(23);8127-36 [PubMed:16291685] [WorldCat.org] [DOI] (P p)
Yancho Devedjiev, Yogesh Surendranath, Urszula Derewenda, Alexandra Gabrys, David R Cooper, Rong-guang Zhang, Lour Lezondra, Andrzej Joachimiak, Zygmunt S Derewenda The structure and ligand binding properties of the B. subtilis YkoF gene product, a member of a novel family of thiamin/HMP-binding proteins. J Mol Biol: 2004, 343(2);395-406 [PubMed:15451668] [WorldCat.org] [DOI] (P p)
Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms. J Biol Chem: 2002, 277(50);48949-59 [PubMed:12376536] [WorldCat.org] [DOI] (P p)
